Mae Questel was born as Mae Kwestel in the Bronx New York City to Simon and Freida Kwestel, she attended Morris High School and studied acting at the American Theatre Wing and with the Theatre Guild. Although she wanted to be an entertainer, her parents, who were Orthodox Jews, actively discouraged her from doing so, at one time forcing her to leave the Theatre Guild school. Nevertheless, at the age of 17 Questel won a talent contest held at the RKO Fordham Theatre in the Bronx by imitating Helen Kane. She was signed by an agent and began performing in vaudeville as an impressionist. Billed as “Mae Questel – Personality Singer of Personality Songs,” she did Fanny Brice, Marlene Dietrich, Eddie Cantor, Mae West, Maurice Chevalier and others, as well as doing animal imitations. She was seen by animator Max Fleischer, who was looking for an actress to provide the voice for his Betty Boop character. Questel’s “Boop-boop-a-doop” routine, done in a style similar to that of the song’s originator, Helen Kane, while at the same time evoking something of the naughty allure of film star Clara Bow, was exactly what Fleischer wanted, and he hired Questel in 1931. She began as one of a number of actresses providing the character’s voice, but soon took over the role exclusively. From 1931 until 1939, Mae Questel provided the voice of Betty Boop in more than 150 animated shorts, the longest run for any actress doing that voice. During the 1930s she released a recording of “On the Good Ship Lollipop” which sold more than two million copies. In 1988, she reprised the role in a cameo appearance in Who Framed Roger Rabbit. Along with her voice work, and occasional on-camera appearance in Paramount shorts, Questel also attended Columbia University, where she studied drama.

Beginning in 1933 Mae Questel also provided the voice for Olive Oyl and Swee’pea in Fleischer’s Popeye animated shorts. She based Olive’s nasal vocal style, and expressions like “Oh, dear!” on the persona of the legendary character actress ZaSu Pitts, and ultimately played the role for more than 20 years. Questel refused to move to Miami, Florida when Fleischer Studios relocated there in 1938, so Margie Hines took over during the Miami years. Questel returned as the voice of Olive Oyl when Paramount Pictures moved the former Fleischer Studios – which became Famous Studios – back to New York. She also filled in for Jack Mercer as the voice of Popeye for a small number of cartoons, made when Mercer was temporarily drawn into war service. When Hanna-Barbera began making the All New Popeye cartoons for television in 1978, Questel auditioned for the role of Olive Oyl but lost out to Marilyn Schreffler. Mae Questel has played a number of small parts, including appearing with Rudy Vallee as Betty Boop in the 1931 short Musical Justice (1931) and as a nurse in The Musical Doctor in 1932.

In 1961 she was seen as a middle-aged bride in Jerry Lewis’ It’s Only Money, one of Fanny Brice’s mother’s card-playing friends at the start of the film Funny Girl in 1968, and as the “Jewish Mama from Hell” in New York Stories in 1989 in Woody Allen’s segment titled “Oedipus Wrecks”; she had earlier sung the song “Chameleon Days” on the soundtrack for Allen’s film Zelig in 1983. Her last non-voice appearance was as the elderly Aunt Bethany in 1989’s National Lampoon’s Christmas Vacation. In 1973, Questel had a role in the short-lived ABC television sitcom The Corner Bar, but she achieved perhaps her greatest visibility in television commercials, notably playing “Aunt Bluebell” in ads for Scott Towels, but also appeared in spots for Playtex, Folger’s Coffee and others. She also appeared on panel shows and in daytime soap operas. Mae Questel had a withered arm; in her on-camera film appearances, she was usually photographed with elbows bent and both hands at her waist or holding an object in the crook of her elbow to make it less obvious that one arm was shorter and smaller than the other. Mae Questel died from complications related to Alzheimer’s disease at the age of 89 in her Manhattan apartment. She was buried in West Babylon, New York’s New Montefiore Cemetery. She had two sons, Robert Balkin, who pre-deceased her, and Richard, who survived her.