Eastman was born in Waterville, New York to George Washington Eastman and Maria Eastman (née Kilbourn), the youngest child, at the 10-acre farm which his parents bought in 1849. He had two older sisters, Ellen Maria and Katie. He was largely self-educated, although he attended a private school in Rochester after the age of eight. His father had started a business school, the Eastman Commercial College in the early 1840s in Rochester, New York, described as one of the first “boomtowns” in the United States, with a rapid growth in industry. As his father’s health started deteriorating, the family gave up the farm and moved to Rochester in 1860. His father died of a brain disorder in May 1862. To survive and afford George’s schooling, his mother took in boarders.  Maria’s second daughter, Katie, had contracted polio when young and died in late 1870 when George was 16 years old. The young George left school early and started working. As George Eastman began to experience success with his photography business, he vowed to repay his mother for the hardships she had endured in raising him.

In 1884, Eastman patented the first film in roll form to prove practicable; he had been tinkering at home to develop it. In 1888, he perfected the Kodak camera, the first camera designed specifically for roll film. In 1892, he established the Eastman Kodak Company, in Rochester, New York. It was one of the first firms to mass-produce standardized photography equipment. The company also manufactured the flexible transparent film, devised by Eastman in 1889, which proved vital to the subsequent development of the motion picture industry.  He started his philanthropy early, sharing the income from his business to establish educational and health institutions. Notable among his contributions were a $625,000 gift in 1901 (equivalent to $17.7 million in present day terms) to the Mechanics Institute, now Rochester Institute of Technology; and a major gift in the early 1900s to the Massachusetts Institute of Technology, which enabled the construction of buildings on its second campus by the Charles River. MIT opened this campus in 1916.

George Eastman never married, although he carried on a long platonic relationship with Josephine Dickman, a trained singer and the wife of business associate George Dickman, and he became especially close to her after the death of his mother, Maria Eastman, in 1907. He was also an avid traveler and had a passion for playing the piano.   His mother, Maria, was his main family for the majority of his life, and her death was particularly crushing to George. Almost pathologically concerned with decorum, he found himself unable for the first time to control his emotions in the presence of friends. “When my mother died I cried all day”, he explained later. “I could not have stopped to save my life”. Due to his mother’s hesitancy and refusal to take his gifts, George Eastman could never do enough for his mother during her lifetime. Thus, after she was gone, George opened the Eastman Theater in Rochester on September 4, 1922, among its features was a chamber-music hall dedicated to her memory: the Kilbourn Theater. And long after that, a rose cutting from her childhood home still flowered on the grounds of the Eastman House.

Eastman was associated with the Kodak company in an administrative and an executive capacity until his death; he contributed much to the development of its notable research facilities. In 1911, he founded the Eastman Trust and Savings Bank. While discouraging the formation of unions at his manufacturing plant, he established paternal systems of support for his employees.  He was one of the outstanding philanthropists of his time, donating more than $100 million to various projects in Rochester; Cambridge, Massachusetts; at two historically black colleges in the South; and in several European cities. In 1918, he endowed the establishment of the Eastman School of Music at the University of Rochester, and in 1921 a school of medicine and dentistry there.

In 1925, Eastman gave up his daily management of Kodak to become treasurer. He concentrated on philanthropic activities, to which he had already donated substantial sums. For example, he donated funds to establish the Eastman Dental Dispensary in 1916. He was one of the major philanthropists of his time, ranking only slightly behind Andrew Carnegie, John D. Rockefeller, and a few others, but did not seek publicity for his activities. He concentrated on institution-building and causes that could help people’s health. From 1926 until his death, Eastman donated $22,050 per year to the American Eugenics Society.

George Eastman donated £200,000 in 1926 to fund a dental clinic in London, UK after being approached by the Chairman of the Royal Free Hospital, Lord Riddell. This was in addition to donations of £50,000 each from Lord Riddell and the Royal Free honorary treasurer. On 20 November 1931, the Eastman Dental Clinic opened in front of Neville Chamberlain and the American Ambassador. The clinic was incorporated into the Royal Free Hospital and was committed to providing dental care for disadvantaged children from central London.

In his final two years, Eastman was in intense pain caused by a disorder affecting his spine. He had trouble standing, and his walk became a slow shuffle. Today, it might be diagnosed as a form of degenerative disease such as disc herniations from trauma or age causing either painful nerve root compressions, or perhaps a type of lumbar spinal stenosis, a narrowing of the spinal canal caused by calcification in the vertebrae. Since his mother suffered the final two years of her life in a wheelchair, she also may have had a spine condition but that is unknown—only her uterine cancer and successful surgery is documented in her health history. If she did have a musculoskeletal disorder, perhaps George Eastman’s spine condition may have been due to a congenital disease, such as ankylosing spondylitis, degenerative disc disease, or a variant of Ehlers–Danlos collagen disorder—conditions known to be inheritable but usually presenting earlier in age. Eastman grew increasingly depressed due to his pain, reduced ability to function, and also since he witnessed his mother’s suffering from pain. On March 14, 1932, Eastman committed suicide with a single gunshot through the heart, leaving a note which read, “My work is done – Why wait? GE.”  His funeral was held at St. Paul’s Episcopal Church in Rochester; he was buried on the grounds of the company he founded at Kodak Park in Rochester, New York.